Sonics VC50 50-watt (1989) User Manual

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USER’S GUIDE
HIGH INTENSITY ULTRASONIC
PROCESSOR
50 Watt Model
TABLE OF CONTENTS
Important Safeguards
SECTION 1 INSTALLATION
Inspection
Electrical Requirements
Installing the Ultrasonic Processor
SECTION II OPERATION
Principles of Ultrasonic Disruption
Functions of Controls, Indications, and Connectors
Preparations for Use
Tuning
Using the Ultrasonic Processor
SECTION III SERVICE INFORMATION
Return of Equipment
SECTION IV OPERATING SUGGESTIONS AND TECHNIQUES
The Ultrasonic Processor supplied with this instruction manual is constructed of the finest
material and the workmanship meets the highest standards. It has been thoroughly tested
and inspected before leaving the factory and when used in accordance with the
procedures outlined in this manual, will provide you with many years of safe and
dependable service.
Rev. 2003
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Summary of Contents

Page 1 - USER’S GUIDE

USER’S GUIDE HIGH INTENSITY ULTRASONIC PROCESSOR 50 Watt Model TABLE OF CONTENTS Important Safeguards SECTION 1 –

Page 2 - IMPORTANT SAFEGUARDS

10 SECTION III – SERVICE INFORMATION Your Ultrasonic Processor was designed to provide you with years of safe and dependable service. Nev

Page 3 - SECTION 1 – INSTALLATION

11 RETURN OF EQUIPMENT It is suggested that an Ultrasonic Processor in need of repair be sent back to the factory. In order to receive pro

Page 4

12 OPERATING SUGGESTIONS AND TECHNIQUES DISRUPTING CELLS The disruption of cells is an important stage in the isolation and preparation of intra

Page 5 - MONITOR sensitivity

13 Microorganisms differ greatly in their sensitivity to ultrasonic disintegration. For example, the most readily disintegrated are the rod-like for

Page 6 - PREPARATION FOR USE

14 If enzymes cannot be used, the following procedures should be considered: Freezing the sample at -70?C overnight, then thawing it in water immedi

Page 7

15 zymolase, glucalase and / or lyticase to produce spheroplasts that are readily lysed may also be useful. To disrupt filamentous fungi, scrape th

Page 8

16 Various methods can be used to measure the efficiency of the disruption. For example, a visual count can be made using a microscope. For greater

Page 9

17 To inhibit sample loss in test tube due to sticking, siliconize the test tube as follows: Wash and dry the test tube thoroughly, coat with silico

Page 10

2IMPORTANT SAFEGUARDS READ BEFORE INSTALLING OR USING THE EQUIPMENT Your Ultrasonic Processor has been designed with safety in mind. However, no de

Page 11

3 SECTION 1 – INSTALLATION INSPECTION Prior to installing the Ultrasonic Processor, perform a visual inspection to detect any evidence of damage,

Page 12

4 INSTALLING THE ULTRASONIC PROCESSOR The Ultrasonic Processor should be installed in an area that is free from excessive dust, dirt, explosive and

Page 13

5 FUNCTIONS OF KEYS, CONTROLS, INDICATORS, AND CONNECTORS POWER SWITCH ON position – energizes the power supply. OFF position – de-energizes th

Page 14

6PREPARATION FOR USE 1. Ensure that the AMPLITUDE is set to OFF. 2. Plug the electrical line cord into the electrical outlet. 3. If the optiona

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7TUNING Tune the power supply in accordance with the following procedures each time a new converter or probe is used. 1. Ensure that the probe is

Page 16

8 USING THE ULTRASONIC PROCESSOR The speed control on an automobile, can, to a certain extent, be compared to an Ultrasonic Processor. The s

Page 17

9 1. Ensure that the power supply is properly tuned. 2. Immerse the probe ½” (1 cm) into the liquid. 3. Set POWER SUPPLY to ON, if fo

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