Sonics VC100 100-watt (1992) User Manual

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USER’S GUIDE
HIGH INTENSITY ULTRASONIC
PROCESSOR
100 Watt Model
TABLE OF CONTENTS
Warranty
Important Safeguards
Low Surface Tension Liquids - Organic Solvents
SECTION 1 INSTALLATION
Inspection
Electrical Requirements
Installing the Ultrasonic Processor
SECTION II OPERATION
Principles of Ultrasonic Disruption
Functions of Controls, Indications, and Connectors
Preparations for Use
Tuning
Using the Ultrasonic Processor
SECTION III SERVICE INFORMATION
Return of Equipment
SECTION IV OPERATING SUGGESTIONS AND TECHNIQUES
The Ultrasonic Processor supplied with this instruction manual is constructed of the finest
material and the workmanship meets the highest standards. It has been thoroughly tested
and inspected before leaving the factory and when used in accordance with the
procedures outlined in this manual, will provide you with many years of safe and
dependable service.
Rev. 2003
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Summary of Contents

Page 1 - USER’S GUIDE

USER’S GUIDE HIGH INTENSITY ULTRASONIC PROCESSOR 100 Watt Model TABLE OF CONTENTS Warranty Important Safeguards Low

Page 2 - WARRANTY

10 USING THE ULTRASONIC PROCESSOR The speed control on an automobile, can, to a certain extent, be compared to an Ultrasonic Processor. The

Page 3 - IMPORTANT SAFEGUARDS

11 1. Ensure that the power supply is properly tuned. 2. Immerse the probe ½” (1 cm) into the liquid. 3. Set POWER SUPPLY to ON, if

Page 4 - INSPECTION

12 SECTION III – SERVICE INFORMATION Your Ultrasonic Processor was designed to provide you with years of safe and dependable service. Neverthe

Page 5

13 RETURN OF EQUIPMENT It is suggested that an Ultrasonic Processor in need of repair be sent back to the factory. In order to receive prompt ser

Page 6 - SECTION II – OPERATION

14 OPERATING SUGGESTIONS AND TECHNIQUES DISRUPTING CELLS The disruption of cells is an important stage in the isolation and preparation of intra

Page 7 - TUNE CONTROL

15 Microorganisms differ greatly in their sensitivity to ultrasonic disintegration. For example, the most readily disintegrated are the rod-like for

Page 8 - PREPARATION FOR USE

16 If enzymes cannot be used, the following procedures should be considered: Freezing the sample at -70?C overnight, then thawing it in water immedi

Page 9

17 zymolase, glucalase and / or lyticase to produce spheroplasts that are readily lysed may also be useful. To disrupt filamentous fungi, scrape th

Page 10

18 Various methods can be used to measure the efficiency of the disruption. For example, a visual count can be made using a microscope. For greater

Page 11

19 To inhibit sample loss in test tube due to sticking, siliconize the test tube as follows: Wash and dry the test tube thoroughly, coat with silico

Page 12

2 WARRANTY Your Ultrasonic Processor is warranted and backed by the manufacturer for a period of three years from the date of shipment agai

Page 13

3IMPORTANT SAFEGUARDS READ BEFORE INSTALLING OR USING THE EQUIPMENT Your Ultrasonic Processor has been designed with safety in mind. However, no de

Page 14

4 CAUTION LOW SURFACE TENSION LIQUIDS – ORGANIC SOLVENTS The probes (solid or with a replaceable tip) are tuned elements that resonate at a specif

Page 15

5 ELECTRICAL REQUIREMENTS The Ultrasonic Processor requires a fused, single phrase 3-terminal grounding type electrical outlet capable of supplyi

Page 16

6 SECTION II – OPERATION PRINCIPLES OF ULTRASONIC DISRUPTION The ultrasonic power supply converts 50/60 Hz line voltage to high frequency electr

Page 17

7 FUNCTIONS OF KEYS, CONTROLS, INDICATORS, AND CONNECTORS POWER SWITCH ON position – energizes the power supply. OFF position – de-energizes the pow

Page 18

8 PREPARATION FOR USE 1. Ensure that the AMPLITUDE is set to OFF. 2. Plug the electrical line cord into the electrical outlet. 3. If the opt

Page 19

9TUNING Tune the power supply in accordance with the following procedures each time a new converter or probe is used. 1. Ensure that the probe is

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