Sonics VCX750 User Manual

U S E R ’ S G U I D EAUTOTUNE SERIESHIGH INTENSITY ULTRASONIC PROCESSORMICROPROCESSOR CONTROLLED (SERIAL NO. "AC" OR HIGHER)500 WATT MODEL •

PREPARATION FOR USE1. Connect the Power Cord to the receptacle on the rear of the processor.2 Plug the Power Cord into a grounded electrical outlet. I

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7No DESCRIPTIONOrderNumber1 Converter Model CV33 CV000332 Four element coupler 630-05583 Stepped top(s) 1/8” (3mm) 630-05354 Booster BHNVCGD5 Probe ½”

8USING THE ULTRASONIC PROCESSORThe speed control on an automobile, can, to a certain extent, be compared to anUltrasonic Processor. The speed control

9Press the I key. The screen will display the power rating and the frequency of theUltrasonic Processor, and the following control parameters.AMPLITUD

101. Using the fittings on top of the converter, circulate dry compressed air through theconverter to cool the converter.2. Immerse the probe into the

113. Depress the START key.4. Using the▲ or ▼ keys, increase or decrease the amplitude as required.TIMER: In the pulsed mode the processing time will

12PULSER: By inhibiting heat build-up in the sample, the pulse function enables safetreatment of temperature sensitive samples at high intensity. In a

13REVIEW: The REVIEW function provides a “window” on the process by displayingvarious operating parameters without process interruption. Pressing theE

14IMPORTANTProper care of the probe is essential for dependable operation. The intensecavitation will, after a long period of time, cause the tip to e

WARRANTYYour Ultrasonic Processor is warranted for a period of three years from thedate of shipment against defects in material and workmanship under

15SECTION III – SERVICE INFORMATIONYour Ultrasonic Processor was designed to provide you with years of safe anddependable service. Nevertheless, becau

16CLEANING INSTRUCTIONSThe generator and converter may be cleaned using a solvent-free cleaning solution(i.e. glass cleaner). Horns and probes should

17SONICS SAFETY CERTIFICATION FORMItems being returned:Please check only one item below:___ The equipment was never used or exposed to any radiologica

18SECTION IV -OPERATING SUGGESTIONS AND TECHNIQUESDISRUPTING CELLSThe disruption of cells is an important method in the field of proteomics and in the

19Single-cell organisms (micro-organisms) consist of a semipermeable, tough, rigidouter cell wall surrounding the protoplasmic membrane and cytoplasm.

20The ability to control the amplitude at the probe tip is a prerequisite for processoptimization. And because each application requires its own set o

21the sample remains trapped in intact cells and, therefore, is unavailable forsubsequent purification. For most samples, thorough disruption can be m

22Detergent cell lysis is sometimes used in conjunction with ultrasonic processing tofacilitate disruption. Detergents break the lipid barrier surroun

23shown to effectively release recombinant proteins located in the cytoplasm of bacteriaand is recommended when lysing mammalian cells. However, this

24When processing a sample with ultrasonics, always immerse the probe deep enoughbelow the surface of the sample to inhibit aerosoling or foaming, foa

IMPORTANT SAFEGUARDSREAD BEFORE INSTALLING ORUSING THE EQUIPMENTYour Ultrasonic Processor has been designed with safety in mind. However, no designcan

25Before each application, place the tip in 100% ethanol and energize the power supplyfor a few seconds to remove any residual substances. If still co

26Fractional protein release, Rp, is calculated using the following equation andmultiplying the result by 100:Rp = Cf – CbCt – CbCf = Free proteinCt =

Standard ½” HornWeight 0.75 lbs. (340 g)Dimensions 5.375" L x .5" Dia.(136 mm x 13 mm)Materials Titanium Alloy Ti-6Al-4VEnvironmentalPolluti

CAUTIONLOW SURFACE TENSION LIQUIDS – ORGANIC SOLVENTSThe probes (solid or with a replaceable tip) are tuned elements that resonate at aspecific freque

1SECTION I – INSTALLATIONINSPECTIONPrior to installing the Ultrasonic Processor, perform a visual inspection to detect anyevidence of damage which mig

2SECTION II – OPERATIONPRINCIPLES OF ULTRASONIC DISRUPTIONThe ultrasonic power supply converts 50/60 Hz line voltage to high frequencyelectrical energ

3Displays prompts and the following control parameters:• Amplitude selected• Output power delivered to the probe in watts, and as percentage of the to

4FUNCTIONS OF KEYS, CONTROLS, INDICATORS, AND CONNECTORS (cont.)REAR PANEL9-pin D-sub connector Connects to external actuation device, and enable powe